Désactiver ponctuellement \beamerdefaultoverlayspecification{}

\documentclass{beamer}

\beamerdefaultoverlayspecification{<+->}

\begin{document}

\begin{frame}
  \frametitle{Titre}
  \begin{columns}[T,onlytextwidth]
    \begin{column}<.->{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Spaghettis
    \end{column}
    \begin{column}<.->{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Doigts crochus
    \end{column}
    \begin{column}<.->{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Ramener à soi un mot dont on a besoin
    \end{column}
    \begin{column}<.->{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Confiance, joie, énergie, force
    \end{column}
\end{columns}

\begin{itemize}
\item test
\item test
\end{itemize}
\end{frame}

\begin{frame}
  \frametitle{Titre}
  {
  \beamerdefaultoverlayspecification{<.->}
  \begin{columns}[T,onlytextwidth]
    \begin{column}{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Spaghettis
    \end{column}
    \begin{column}{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Doigts crochus
    \end{column}
    \begin{column}{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Ramener à soi un mot dont on a besoin
    \end{column}
    \begin{column}{.23\textwidth}
      \centering
      \includegraphics[width=\linewidth]{example-image-duck}

      Confiance, joie, énergie, force
    \end{column}
\end{columns}
}

\begin{itemize}
\item test
\item test
\end{itemize}
\end{frame}

\end{document}